low binding 96 well plates Search Results


96 well protein binding plates  (Revvity)
92
Revvity 96 well protein binding plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
96 Well Protein Binding Plates, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/96 well protein binding plates/product/Revvity
Average 92 stars, based on 1 article reviews
96 well protein binding plates - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier
low binding 384 well plate  (Eppendorf AG)
94
Eppendorf AG low binding 384 well plate
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
Low Binding 384 Well Plate, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/low binding 384 well plate/product/Eppendorf AG
Average 94 stars, based on 1 article reviews
low binding 384 well plate - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
microtiter plates  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology microtiter plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
Microtiter Plates, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtiter plates/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
microtiter plates - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
cell binding v bottom 96 well plate  (Greiner Bio)
96
Greiner Bio cell binding v bottom 96 well plate
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
Cell Binding V Bottom 96 Well Plate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cell binding v bottom 96 well plate/product/Greiner Bio
Average 96 stars, based on 1 article reviews
cell binding v bottom 96 well plate - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
dna low bind 96 well plate  (Eppendorf AG)
99
Eppendorf AG dna low bind 96 well plate
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
Dna Low Bind 96 Well Plate, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna low bind 96 well plate/product/Eppendorf AG
Average 99 stars, based on 1 article reviews
dna low bind 96 well plate - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
96 well immunoassay high binding u bottom plates  (Greiner Bio)
99
Greiner Bio 96 well immunoassay high binding u bottom plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
96 Well Immunoassay High Binding U Bottom Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/96 well immunoassay high binding u bottom plates/product/Greiner Bio
Average 99 stars, based on 1 article reviews
96 well immunoassay high binding u bottom plates - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
96 well protein lowbind plate  (Eppendorf AG)
99
Eppendorf AG 96 well protein lowbind plate
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
96 Well Protein Lowbind Plate, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/96 well protein lowbind plate/product/Eppendorf AG
Average 99 stars, based on 1 article reviews
96 well protein lowbind plate - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
low binding 6 well plate  (Greiner Bio)
96
Greiner Bio low binding 6 well plate
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
Low Binding 6 Well Plate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/low binding 6 well plate/product/Greiner Bio
Average 96 stars, based on 1 article reviews
low binding 6 well plate - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
96 well fluoroimmunoassay fia black plates  (Greiner Bio)
99
Greiner Bio 96 well fluoroimmunoassay fia black plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
96 Well Fluoroimmunoassay Fia Black Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/96 well fluoroimmunoassay fia black plates/product/Greiner Bio
Average 99 stars, based on 1 article reviews
96 well fluoroimmunoassay fia black plates - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
flat bottom polypropylene non binding 96 well plates  (Greiner Bio)
99
Greiner Bio flat bottom polypropylene non binding 96 well plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
Flat Bottom Polypropylene Non Binding 96 Well Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flat bottom polypropylene non binding 96 well plates/product/Greiner Bio
Average 99 stars, based on 1 article reviews
flat bottom polypropylene non binding 96 well plates - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
96 well medium binding lumitrac 200 plates  (Greiner Bio)
99
Greiner Bio 96 well medium binding lumitrac 200 plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
96 Well Medium Binding Lumitrac 200 Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/96 well medium binding lumitrac 200 plates/product/Greiner Bio
Average 99 stars, based on 1 article reviews
96 well medium binding lumitrac 200 plates - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
sterile low binding 24-well plates  (Corning Life Sciences)
90
Corning Life Sciences sterile low binding 24-well plates
HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to <t>96-well</t> plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.
Sterile Low Binding 24 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sterile low binding 24-well plates/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
sterile low binding 24-well plates - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to 96-well plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.

Journal: Journal of Virology

Article Title: The High Content of Fructose in Human Semen Competitively Inhibits Broad and Potent Antivirals That Target High-Mannose Glycans

doi: 10.1128/JVI.01749-19

Figure Lengend Snippet: HIV-1 infection and inhibition in human seminal plasma. (A) Dynamics of SP cytotoxicity. Cf2Th CD4+ CCR5+ cells were incubated with whole hSP from different donors or with sSP(+Fr) for the indicated times, washed, and cultured in DMEM for 36 h. Cell viability was measured by an ATP-based assay. (B) Cf2Th CD4+ CCR5+ cells were added to 96-well plates at the indicated densities. Samples were then incubated at 37°C for 75 min with DMEM or a sample containing 77.5% hSP and 22.5% Tris-saline buffer. All samples were then washed and further processed as described in Fig. 3C, and viability was measured after 20 or 36 h. Viability values are corrected for the number of cells seeded in each well. (C) Fructose concentrations measured by the resorcinol and indole assays. Fructose standards were diluted in Tris-buffered saline (pH 7.5). (D) Cell viability (after 75 min of exposure) and fructose content (quantified by resorcinol) in 59 hSP samples from different donors were measured. Since samples were tested in separate experiments, values are reported as a percentage of the viability in the DMEM control included in each assay. CAVD, congenital absence of the vas deferens. (E) Infection by virus containing strain 1053-07 or WEAU Envs in DMEM, sSP(+Fr), and three pools of donor hSP using the protocol shown in Fig. 3C. Cell viability was measured in the same experiment. (F) HIV-1 inhibition in hSP. Infection by virus that contains the WEAU Env was measured in DMEM and whole pooled hSP from 10 donors (pool C) in the presence of GRFT (60 nM), 2G12 (20 μg/ml), PGT121 (3 μg/ml), b12 (15 μg/ml), maraviroc (1 μM), tenofovir (2 μM), or T-20 (6 μM). Cell viability after exposure to hSP pool C was measured in the same experiment and was 59.8% of that measured in DMEM. Error bars, SEM. P values were determined by a two-tailed t test. *, P < 0.05; **, P < 0.005. (G) GRFT inhibition of virus containing the 1053-07 Env in hSP samples from different donors. Viability values are expressed as a percentage of those measured in samples incubated with DMEM. (H) (Left) Immobilized viruses containing the WEAU Env were treated as shown, using GRFT (50 nM), 2G12 (20 μg/ml), or PGT121 (3 μg/ml). Entry was then halted using trypsin and T-20, and infectivity was measured 3 days later. (Right) The viability of samples similarly treated in the same experiment was measured after 36 h. (I) Infection and GRFT inhibition in fresh and thawed hSPs. Samples were collected from donors and either subjected to one freeze-thaw cycle (frozen) or left at room temperature (fresh) for 4 h, before they were used for infection assays in the absence or presence of GRFT.

Article Snippet: To measure infection in synthetic or human SP, viruses suspended in PBS were attached to 96-well protein-binding plates (PerkinElmer) by spinoculation at 2,000 × g for 2 h at 10°C.

Techniques: Infection, Inhibition, Incubation, Cell Culture, ATP Assay, Saline, Virus, Two Tailed Test